Document Type : Articles
Authors
College of Agriculture - University of Tikrit, Tikrit, Iraq
Abstract
Twenty-eight isolates of Lactobacillus were isolated from the rhizosphere of pea cultivated in the fields of five districts in Salah al-Din, which included: Tikrit, Al-Alam, Al-Sharqat, Samarra and Al-Baiji. The isolates identified according to molecular diagnosis using the analysis of the 16S rRNA sequences and were divided into five species: 7 Isolates of L. fermentum, 3 isolates of L. casei, 9 isolates of L. plantarum, 4 isolates of L. herbarum and 5 isolates of L. paralimentarius. Antagonism tests were conducted for these isolates against the pathogen Pythium aphanidermatum, the highest rate of inhibition was by isolates of bacteria L. paralimentarius 1981 reached 2.7 mm, followed by isolate L.plantarum 1982 with an inhibition rate of 2.5 mm. In the antagonism test using bacterial filtrate of L. paralimentarius 1981, the concentration of 30% was the highest in inhibiting the pathogenic fungus, as the fungus colony diameter reached 3.22 cm, followed by the concentration of 20 and 25%, as the colony diameter reached 4.47 and 3.68 cm, respectively. L. paralimentarius 1081 was recorded in the databases of the World Genetic Bank within the accession number MW149434.1. This is the first record of this bacteria in Iraq. L. paralimentarius 1081 showed the highest production of the antibacterial active substances including siderophores, total phenols and bacteriocin reached to 61.24%, 1.05 mg/ml and 18.02 mg/ml, respectively, as well as pH decreased to 4.5. The results also showed there are a strong correlation of pathogenic fungus inhibition with high concentrations of siderophores, total phenols, bacteriocin and low pH in the bacterial filtrate.
Keywords
- Alkurtany, A., Hassan, A. A., and Yousef, H. (2018a). Biochemical and Molecular Identification of P.fluorescens Isolated from Iraqi Soils and Evaluation of Their Efficacy in Production of Plant Growth Promoting Compounds and Inhibitor Compounds for Macrophomina phaseolina. Tikrit Journal For Agricultural Sciences, 18(2), 143-157.
- Alkurtany, A., Hassan, A. A., and Yousef, H. (2018b). Evaluation of fluorescens Pseudomonas efficacy for promoting corn Zea mays. Growth and induction of it systematic resistance against charcoal rot disease. Tikrit Journal For Agricultural Sciences, 18(1), 137-149. http://tujas.tu.edu.iq/index.php/ph/article/view/24
- Al-Rawi, K. Mahmoud and Khalaf Allah, A. Muhammad. (1980) . Design and analysis of agricultural experiments. Dar Al-Kutub for Printing and Publishing, University of Mosul.
- Cadet J, Wagner JR. (2014) . Oxidatively generated base damage to cellular DNA by hydroxyl radical and one-electron oxidants: similarities and differences. Arch. Biochem. Biophys. (1): 557:47-54.
- Caly, D. L., Chevalier, M., Flahaut, C., Cudennec, B., Al Atya, A. K., Chataigne, G., et al. (2017). The safe enterocin DD14 is a leaderless two-peptide bacteriocin with anti-Clostridium perfringens activity. Int. J. Antimicrob. Agents 49, 282– 289.
- Canpolat, E., M.D. Müzeyyen, D. Sibel and U.S. Çiğdem. (2018). Antifungal activity of some lactic acid bacteria against several soilborne fungal pathogens isolated from strawberry plants. Turkish Journal of Agriculture - Food Science and Technology, 6: 1163-1167.
- Desmazeaud M INRA, Je Piard (1991), Station de Recherches Laitières, Unite de Microbiologie, 78350 Jouy-en-Josas, France .
- Elshahawy A., Abouelnasr M.H., Lashin M.S., Darwesh M.O., (2018) . First report of Pythium aphanidermatum infecting tomato in Egypt and its control using biogenic silver nanoparticles . Journal of Plant Protection Research . 58,(2): 137–151.
- Fijan, S. (2014). Microorganisms with claimed probiotic properties: An overview of recent literature. Int. J. Environ. Res. Public Health 11:4745–4767.
- Ghadoliya M.K., Kaswan, R. K., Shyam,R., Jain, S.K., Gupta, A. K., Verna, A., Agnihotri, P. and J. Ajay. (2020).Innovative Research on Science, Humanities, Engineering As Biological Control Agent .pp. 268.
- Ghosh S. ., Sujoy P., and Niloy C.(2015).The Qualitative And Production By Some Microorganisms And Effect Different Media On Its Production . Int. J. Chem. Sci.: 13(4):1621-1629.
- Gourmelon, V., Maggia, L., Powell, J. R., Gigante, S., Hortal, S., Gueunier, C., Letellier, K., Carriconde, F. (2016). Environmental and Geographical Factors Structure Soil Microbial Diversity in New Caledonian Ultramafic Substrates: A Metagenomic Approach . Plos one, 11 (12): 1-25.
- Hassan , A. A., Saba, A. E., Yousef, H. M. .(2017). Isolation and Identification of Active Compounds From Pseudomonas fluorescens Isolated from Iraqi Soils and Evaluation of Their Efficiency on Growth of Pathogenic Fungus Macrophomina phaseolina. Journal of Tikrit University For Agriculture Sciences. 169-181.Proceedings of the sixth Agricultural Scientific Conference. 28-29 March 2017 – College of Agriculture – University of Tikrit.
- Hassan, Abdullah A., Ahmed, Waleed Khaled. (2015). Evaluation of Compost and Biological Control Efficiency Agents in Charchol Rot Disease Caused by Macrophomina phaseolina (Tassi) Codi on Corn. Tikrit Journal for Agricultural Sciences. 15 (3): 77-99. http://tujas.tu.edu.iq/index.php/ph/article/view/256
- Holt, J. C. ; Krieg, N.R. ; Sneath, P.H.A. : Stahley, J. T. and Willa, S. T.(1994). Berge's manual of determinative bacteriology,9th ed. USA.
- Husain, A., Z. Hassan, N. Huda-Faujan and M.N. Lani. (2017). Antifungal activity of Lactic acid bacteria isolated from soil rhizosphere on Fusarium species infected chilli seeds. American Scientific Research Journal for Engineering, Technology, and Sciences, 29: 182-202.
- Jay Ram L., Carolyne D., Andre A. S., Marie-Helène R., Jean-Pierre S., Vincent C., Antoine M. and Jean-Noel A. (2017). Integrated management of damping-off diseases. A review. Agronomy for Sustainable Development, Springer Verlag /EDP Sciences/INRA.37 (2):1- 25.
- Kanatani, K., Oshimura, M. and Sano, K. (1995) Isolation and characterization of acidocin A and cloning of the bacteriocin gene from Lactobacillus acidophilus. Applied and Environmental Microbiology 61, 1061–1067.
- Kirithiga, A. and Namasivayam , K.R. (2010). Effect of Formulation of Effective Microorganism(EM) on Post Treatment Persistence, Microbial Density and Soil Macronutrients. Recent Research in Science Technology. 2(5): 102-106.
- Lamont John R., Wilkins, O.,Bywater-Ekegard, M.and Donald L. Smith. (2017).From yogurt to yield: Potential applications of lactic acid bacteria in plant production Soil Biology & Biochemistry. 7: 1-9.
- Loper, J. E. and Henkels, M. D.(1997). Availability of iron to P. fluorescens in rhizosphere and bulk soil evaluated with an ice nucleation reporter gene. Applied and Environmental Microbiology.63:99–105.
- Lv, X., Ma, H., Lin, Y., Bai, F., Ge, Y., Zhang, D., and Li, J. (2018). Antifungal activity of Lactobacillus plantarum C10 against Trichothecium roseum and its application in promotion of defense responses in muskmelon (Cucumis melo L.) fruit. Journal of Food Science and Technology.55(9):3703–3711.
- Mac Faddin,J.F.(2000).Biochemicaltests for identification of medical microbiology. Lippincott Williams and Wilkins Publisher.
- Mahadevan, A. and Sridhar, R.(1986). Methods in physiological plant pathology. Sivakami publications, Indira nagar, India.
- Majeed Z.H. and Al-Shammari H.R.(2016). Efficiency of Lactic Acid Bacteria as biological control agents against some Fungi . Al-Mustansiriyah Journal of Science .27(2):22-32.
- Martins M., McCusker M.P., Viveiros M., Couto I., Fanning S., Pagès J.M., Amaral L.(2013). A Simple Method for Assessment of MDR Bacteria for Over-Expressed Efflux Pumps. Open Microbiol J. 7:72-82.
- McKellar, R. C., Piyasena, P. and Mohareb, E. (2003). Inactivation of microbes using ultrasound: A review. International Journal of Food Microbiology.87:207–216.
- Pedraza R., Motok J., Tortora M., Salazar S., Diaz-Ricci, J. (2007). Natural occurrence of Azospirillum brasilense in strawberry plants. Plant Soil 295:169–178.
- Rouse S., Carlos Canchaya and Douwe van Sinderen.(2008) . Lactobacillus hordei sp. nov., a bacteriocinogenic strain isolated from malted barley . International Journal of Systematic and Evolutionary Microbiology.22:1-58.
- Seddik, H. A., Bendali, F., Gancel, F., Fliss, I., Spano, G., and Drider, D. (2017). Lactobacillus plantarum and its probiotic and food potentialities. Probiotics Antimicrob. Proteins. 9:111–122.
- Sharma, P. (2015).Evaluation of diseases control and plant growth promoting potention of biocontrol agents on Pisum sativum and comparison of their activity with popular chemical control agent bcarbendazin. Journal of Toxicology and environmental Health Sciences: 3(5):127-138 .
- Shrestha A., Kyu-up Choi, Chun Keum Lim , Jang Hyun Hur and Seayoull Cho. (2009). Antagonistic Effect of Lactobacillus sp. Strain KLF01 Against plant pathogenic Bacteria Ralstonia Solanacearum . The Korean Journal of pesticide Science. 3(13): 45-53 .
- Sivasakthi, S., G., Usharani and Saranraj, P.(2014). Biocontrol potentiality of plant growth promoting bacteria (PGPR) - Pseudomonas fluorescens and Bacillus subtilis: A review. Afr. J. Agric. Res. 9:1265-1277
- Stoyanova L. G., Ustyugova, E. A. and Netrusov, A. I..(2012). Antibacterial Metabolites of Lactic Acid Bacteria: Their Diversity and Properties. Biokhimiya in Mikrobiologiya. 48(3):259–275 .
- Van Buyten, E., and Höfte, M. (2013). Pythium species from rice roots differ in virulence, host colonization and nutritional profile. BMC Plant Biology, 13(1): 203. doi:10.1186/1471-2229-13-203
- Vuyst De, De Preter, V., Vanhoutte, T., Huys, G., Swings, J., , L., Rutgeerts, P., and Verbeke, K. (2007). Effects of Lactobacillus casei Shirota, Bifidobacterium breve, and oligofructose-enriched inulin on colonic nitrogen-protein metabolism in healthy humans. American Journal of Physiology-Gastrointestinal and Liver Physiology, 292(1):G358–G368. doi:10.1152/ajpgi.00052.2006
- Whipps, J. M. (2001). Microbial interactions and biocontrol in the rhizosphere. Journal of Experimental Botany. 52:487–511.
- Zebboudj N , Wassim Y., Hamini-Kadar, N., Kihal, M. and Henni, J. E. (2014). Laboratory of Applied Microbiology, Department of Biology, Faculty of Nature Sciences and Life, University of Oran. BP 16, Es-Senia 31100, Oran, Algeria. http://dx.doi.org/10.12692/ijb/5.9.99-106 .
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