Document Type : Articles
Authors
1 Department of Horticulture and Landscape , College of Agriculture , Tikrit University, Iraq
2 Department of Biology, College of Science, Tikrit University, Iraq
Abstract
The experiment was carried out at the Plant Tissue Culture Laboratory of the Department of Horticulture and Landscape - College of Agriculture - Tikrit University.The experiment aimed to know the effect of Brassinolide(BRs) on the induction of callus formation of the basil plant Ocimum basilicum L and its estimated content of active substrates.The basil plant seeds were sowed in the solid medium Murashige and Skoog ( without plant growth regulators). True leaves were taken and grown on Murashige and Skoog medium supplied with concentrations of (0.0, 0.5, 1.0, 1.5, and 2.0) mg.L-1 of 2,4-D mixed with a concentration of 0.5 mgL-1 of Kin. After 4 weeks of planting, the results showed variation in callus generation between the concentrations of 2,4-D used.The concentration of 2.0 mg L-1 of 2,4-D mixed with 0.5 mg L-1 Kin gave the highest percentage of callus formation reaching 100%, the highest fresh weight amounting to 1.0325 g, and the highest callus volume (+++). The results of planting callus using different concentrations of Brassinolide (0.0, 0.01, 0.1, and 0.2) mg L-1 showed that callus grown on Murashige and Skoog medium supplied with a concentration (0.1 mg L-1) gave the highest fresh weight (0.6664 g)and dry weight (0.0710 g) of callus, While the concentration(0.2 mg L-1) of Brassinolide gave the highest concentration of the Caffeic acid, Luteolin, Rosemaric acid, Gallic acid andFerulic acid(15.49, 11.04, 16.90, 20.24, 7.90) ppm, respectively
Keywords
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