Document Type : Articles
Authors
- Thamer Al-Shuwaili 1
- Farhan Jasim Mohammed Al-Behadili 2
- Mushtak Talib Mohammadali 2
- Zainab Mohammed Khalaf 3
- Beibei Li 4
- Yonglin Ren 5
1 Department of plant protection, University of Kerbala, Kerbala,56001, Iraq
2 Department of plant protection, College of Agriculture, University of Misan, Misan 62001, Iraq.
3 Department of plant protection, College of Agriculture, University of Misan
4 Department of plant biosecurity, Murdoch University, Murdoch, 6150, Australia
5 College of Environmental and Life Sciences, Murdoch University, Perth 6150, Australia
Abstract
The objective of this study is to analyze Trogoderma variabile metabolism fed on various host grains (Rice, maize, wheat, oats, barley and canola) using GC-MS. Also, hydrocarbons profiling is suggested as a chemo-taxonomical marker/ tool to identify species of insect and especially when they are morphological quite similar like T. granarium. Six different commodities were the rearing substrates of T. variabile. To prepare the samples, insects were treated with acetonitrile. A method called DI-SPME was utilized, followed by GC-MS for analyzing and categorizing compounds. Furthermore, the specific type of grain that the insects fed on had a notable impact on the chemical compounds detected in Trogoderma variabile different larvae stages, including fatty acids and hydrocarbons. However, there were 17,16 , 14,16,14 and 13 compounds extracted form third larvae instar reared on barley, wheat,canola, maize, rice and oats respectively. in comparison to sixth larvae instar, results showed that there were 13,13,21,21,15 and 14 compounds from the larvae reared on oats and barley, wheat,canola, maize, rice and oats respectively.
Keywords
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