Document Type : Articles

Authors

Natural Resources Department, College of Agricultural Engineering Sciences, University of Sulaimani, Sulaimani – Iraq

Abstract

Using microbial bio fertilizer has been widely used in recent years because it improves the plant growth and nutrients uptake  A plastic pot experiment was carried out under plastic house at the College of Agricultural Engineering Sciences, University of Sulaimani, Bakrajo, from November 2021 to May 2022 to study the effect of co-inoculation of bacteria A. brasilense and B. megaterium bio fertilizer and their interaction on growth responses of wheat (Triticum aestivumn L.) at various  P levels (0, 40, 80,120, and 160 kg P ha-1). The experiment was performed in a factorial experiment with completed randomized design (CRD) in a silty clay soil with three replications. Before harvesting chlorophyll, intensity was measured, and after that plants were harvested. Results showed that co-inoculation was significantly effect on increasing chlorophyll intensity and plant dry biomass on wheat plant and nutrient uptake. The maximum value was 36.54 spad and 19.82 g pot-1 recorded at (160 kg P ha-1) respectively, while the maximum value of wheat shoot N, and Zn nutrients contend was 27.97 g kg-1 and 45.44 µg g-1 respectively recorded at (160 kg P ha-1), while the maximum value for P, K and Fe was 5.33 g kg-1, 26.43 g kg-1 and 301.74 µg g-1 recorded at (120 kg P ha-1 ). But when the soil inoculated with A. brasilense significantly increased chlorophyll intensity and plant dry biomass   compared with control. The maximum value was 28.50 spad and 19.22 g pot-1 recorded at (160 kg P ha-1), while the highest value for wheat shoot N, P, K Fe and Zn nutrients contend were 21.10 g kg-1, 2.80 g kg-1, 15.77 g kg-1, 201.56 µg g-1 and  26.45  µg g-1    respectively recorded at (160 kg P ha-1), and when the soil inoculated with B. megaterium increased chlorophyll intensity and plant dry biomass  compared with control. The highest value was 18.50 spad and 16.31 g pot-1 recorded at (160 kg P ha-1). while the maximum value for wheat shoot N, P, K and Fe nutrients were 20.26 g kg-1, 4.43 g kg-1, 17.83 g kg-1 and 276.74 µg g-1 respectively recorded at (160 kg P ha-1), while the maximum value for Zn was 32.85 µg g-1 recorded at (120 kg P ha-1).

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