Document Type : Articles

Authors

a:1:{s:5:"en_US";s:25:"Ministry of agricultural ";}

Abstract

 The research was conducted at the Khalis cows station  in Al-Khalis district - Diyala Governorate and the central laboratory of the Faculty of Agriculture - University of Tikrit for the period from 13/12/2020 to 1/2/2022, a sample of 63 Holstein cows, with the aim of extracting the genetic material and determining the genetic polymorphism of the SCD1 gene and its relationship to reproductive traits in Holstein cows, studying the percentage of distribution of genetic structures in the cows that were studied, the allele frequency and calculating the value of chi-square (χ2) were determined. The genotypes  frequencies for the SCD1 gene in the studied cow samples were 47.62, 46.03 and 6.35% for AA, AV and VV genotypes respectively. The value of the chi-square (χ2) was highly significant (P < 0.01). The frequency of allele A  and V were 0.71 and 0.29 respectively. Some reproductive traits were significantly affected by the different genotypes of the SCD1 gene. Cows with the genotype AA achieved the highest  Days Open  (106.70 ± 5.84 days), while the genotypes VV and AV were 105.93 ± 14.25 and 92.82 ± 5.89 days respectively. The cows with AA genotype was the highest calving interval  (389.77 ± 5.90 days), while the cows of the VV and AV genotypes reached 386.75 ± 14.39 and 374.27 ± 5.96 days respectively. It can be concluded by studying the genetic polymorphism of the SCD1 gene that it is possible to improve the  reproductive traits  in cows. We also recommend applying the study to a larger sample for several seasons and different locations of the gene, while studying more economic characteristics to give more accurate results for the application of the selection and exclusion strategy.

  1. Bernard, L.,Leroux, C. and Chilliard, Y. (2013). Expression and Nutritional Regulation of Stearoyl-CoA Desaturase Genes in the Ruminant Mammary Gland: Relationship with Milk Fatty Acid Composition. In Stearoyl-CoA Desaturase Genes in Lipid Metabolism; Springer: New York, NY, USA, pp. 161–193.
  2. Bernard, L., Leroux, C., Hayes, H., Gautier, M., Chilliard, Y. and Martin, P. (2001). Characterization of the caprine stearoyl-CoA desaturase gene and its mRNA showing an unusually long 3′-UTR sequence arising from a single exon. Gene., 281(1–2):53–61.
  3. Bouwman, A. C., Bovenhuis, H., Visker, M. H. and Van Arendonk, A. (2011). Genome-wide association of milk fatty acids in Dutch dairy cattle. BMC Genetics,12:43.
  4. Carvajal AM, Huircan P, Dezamour JM, Subiabre I, Kerr B, Morales R. and Ungerfeld, E.M. (2016). Milk fatty acid profile is modulated by DGAT1 and SCD1 genotypes in dairy cattle on pasture and strategic supplementation. Genet. Mol. Res., 15:7057.
  5. Chagas L.M., Bass J.J., Blache D., Burke C.R., Kay J.K., Lindsay D.R., Lucy M.C., Martin G.B., Meier S., Rhodes F.M., Roche J.R., Thatcher W.W. and Webb R. (2007). Invited review: new perspectives on the roles of nutrition and metabolic priorities in the subfertility of high-producing dairy cows. J. Dairy Sci., 90:4022–4032.
  6. Dash. S. K., Gupta, A. K., Manoj, M., Kumar, V., Shivhre, P. R. and Valsalan, J. (2018). Analysis of lifetime performance in Karan Fries Cattle. Indian J.of Anim. Res., 52 (5): 761–767.
  7. Duncan, D.B. (1955). Multiple Rang and Multiple F-test. Biometrics. 11: 4-42.
  8. FAO. (2009). The state of food and agriculture. Rome, Italy, pp. 1-180.
  9. Fox, P. F. (2003). Milk proteins: general and historical aspects. Pages 1-48 in Advanced dairy chemistry: proteins. Vol. 1. P. F. Fox and P. L. H. McSweeney, ed. Kluwer Academic/Plenum Publishers, New York.
  10. Kesek, M. M., Smołucha, G. and Zielak-Steciwko, A. E. (2017). Acetyl-CoA Carboxylase α and Stearoyl-CoA Desaturase Genes Polymorphism and their Influence on Fatty Acid Profile in Milk of Polish Holstein-Friesian Cows. Annals of Animal Science, 17(4), 993–1006.
  11. Kovalchuk, S.N., Arkhipova, A. L. and Klimov, E. A. (2020). Development of real-time PCR assay for genotyping SNP rs41255693 in cattle SCD gene. AIMS Agriculture and Food, 5(1): 14–19.
  12. Kulig, H., Kowalewska-uczak, I., Zukowski, K. and Kunicka, M. (2013). SCD1 SNP in relation to breeding value of milk production traits in Polish Holstein-Friesian cows. Acta Sci. Pol., 12: 41–48.
  13. Li, Y., Zhou, H., Cheng, L., Zhao, J. and Hickford, J. (2020). Variation in the stearoyl-CoA desaturase gene (SCD) and its influence on milk fatty acid composition in late-lactation dairy cattle grazed on pasture. Arch. Anim. Breed, 63: 355–366.
  14. Lopez- Gatius, F., Yaniz, J. and Madriles-Helm. D. (2003). Effects of body condition score and score change on the reproductive performance of dairy cows: a meta-analysis. Theriogenology,59: 801 - 812.
  15. SAS. (2018). Statistical Analysis System, User's Guide. Statistical. Version 9.1th ed. SAS. Inst. Inc. Cary. N.C. USA.
  16. Sejian V, Gaughan, JB., Bhatta, R. and Naqvi, SMK. (2016). Impact of climate change on livestock productivity. Feedipedia-Animal Feed Resources Information System - INRA CIRAD AFZ and FAO, p. 1-4.
  17. Strittmatter, P., Spatz, L., Corcoran, D., Rodgers, M., Setlow, B. and Redline, R. (1974). Purification and properties of rat liver microsomal stearoyl coenzyme A desaturase. Proceedings of the National Academy of National Sciences, USA. 71,4565-4569.
  18. Taniguchi, M., Utsugi, T., Oyama, K., Mannen, H., Kobayashi, M., Tanabe, Y., Ogino, A. and Tsuji, S. (2004). Genotype of stearoyl-CoA desaturase is associated with fatty acid composition in Japanese Black cattle. Mamm .Genome,14:142-148.