Document Type : Articles

Authors

a:1:{s:5:"en_US";s:73:"College of Agricultural Engineering Sciences / University of Sulaimaniyah";}

Abstract

The fig (Ficus carica L.) is a fruit tree that is important in the Mediterranean region, and thus genetic improvement has become an important field of research for better crops, with information on this species, particularly its genetic variability correlated to morphological traits of figs, for this purpose, the cultivars and wild types distributed in Sulaymaniyah province were investigated. Samples from 66 fig accessions were collected, 15 primers for ISSR markers were used to describe genetic variation, with 12 morphological traits. Analysis of variance recorded highly significant differences concerning plant morphological and pomological traits in addition to genetic diversity. The highest values for shoot length (79.959 cm), internode diameter (15.563 mm), leaf length (28.183 cm), leaf width (28.480 cm), leaf petiole length (13.397 cm) and leaf petiole diameter (18.360 mm) recorded in AC17, AC41, AC20, AC20, AC17 and AC24, respectively. However, the lowest values (11.120 cm, 4.340 mm, 10.910 cm, 9.813 cm, 3.987 cm and 2.323 mm) for the mentioned traits were recorded in AC14, AC12, AC12, AC12, AC37 and AC52, respectively. The highest values for fruit weight (63.447 g), fruit length (46.960 mm), fruit thickness (60.420 mm), fruit stalk length (29.887 mm), fruit stalk diameter (10.433 mm) and ostiole diameter (8.717 mm) were given by AC03, AC22, AC54, AC58, AC58 and AC14, successively. Whereas the lowest values (4.483 g, 14.770 mm, 18.497 mm, 2.373 mm, 2.533 mm and 2.557 mm) were observed in AC37, AC37, AC12, AC08, AC25 and AC30, successively. The first two principal components analysis (PCA) described 49.15% of the total quality variance. ISSR marker produced 197 polymorphic bands. The genetic diversities ranged as (0.883 to 0.980) and polymorphism information content (PIC) ranged as (0.878 to 0.979), with 100% polymorphism levels. The fig accessions classified into 10 clusters by dendrongram created by ward method. The results indicated that natural fig populations in this region provide a rich genetic resource for fig germplasms, and significant genetic variation across accessions originating from different populations, as well as the marker was informative for genetic variability detection in the collections. The findings of this study could support in the conservation and further utilization of fig germplasm.

  1. Abd El-Aziz MH, Mohamed SY, Magwaid HE, 2019. Molecular and phytochemical assessment for some seedy strains of Alamar apricot rootstock under salinity stress. Egyptian Journal of Basic and Applied Sciences 6 (1): 173–186. https://doi.10.1080/2314808X.2019.1690358
  2. Abdel Hameed UK, Abdelaziz K, El-Sherif N, 2020. Genetic diversity of grapevine (Vitis vinifera L.) cultivars in Al-Madinah Al-Munawara based on molecular markers and morphological traits. Bangladesh Journal of Plant Taxonomy 27 (1): 113–127. https://doi.10.3329/bjpt.v27i1.47573
  3. Abdelsalam NR, Awad RM, Ali HM, Salem MZM, Abdellatif KF, Elshikh FS, 2019. Morphological, pomological, and specific molecular marker resources for genetic diversity analyses in fig (Ficus carica L.). HortScience 54 (8): 1299–1309. https://doi.10.21273/HORTSCI14091-19
  4. Ahmed AA, Qadir SA, Tahir NA, 2022. CDDP and ISSR markers-assisted diversity and structure analysis in Iraqi Mazu (Quercus infectoria Oliv.) accessions. All Life 15 (1): 247–261. http://doi.10.1080/26895293.2022.2042401
  5. Al-Ameri AA, Al-Qurainy F, Gaafar AZ, Khan S, Nadeem M, 2016. Molecular Identification of Sex in Phoenix dactylifera Using Inter Simple Sequence Repeat Markers. Hindawi Publishing Corporation, BioMed Research International 2016. http://doi:10.1155/2016/4530846
  6. Ali SH, 2019. Characterization of Microsatellite Loci in different Fig (Ficus carica L.) Landraces in Duhok and Erbil Provinces in Kurdistan Region-Iraq. Zanco Journal of Pure and Applied Sciences 31 (2): 48-56..
  7. Baraket G, Chatti K, Saddoud O, Abdelkarim AB, Mars M, Trifi M, Hannachi AS, 2011. Comparative assessment of SSR and AFLP markers for evaluation of genetic diversity and conservation of fig, Ficus carica L., genetic resources in Tunisia. Plant Molecular Biology Reporter, 29, pp.171-184.
  8. Bhavana P, Chaoudhary AK, Santosh KB, Maurya S, Choudhary JS, Naik SK, Reshma S, Nawe A, 2019. Genetic diversity and principal component analysis for yield characters of Pigeonpea landraces of Eastern India. International Journal of Chemical Studies 6: 547–551.
  9. Caliskan O, Bayazit S, Ilgin M, Karatas N, Ergul A, 2018. Genetic diversity and population structure in caprifigs (Ficus carica var. caprificus) using SSR markers. Spanish Journal of Agricultural Research 16 (3): e0703. http://doi:10.5424/sjar/2018163-11662
  10. Çalişkan O, Polat AA, 2012. Morphological diversity among fig (Ficus carica L.) accessions sampled from the Eastern Mediterranean Region of Turkey. Turkish Journal of Agriculture and Forestry 36 (2): 179–193.
  11. Chithiraichelvan R, Kurian RM, Awachare CM, Laxman RH, 2017. Performance of Fig (Ficus carica L.) Under Different Planting Densities. International Journal of Current Microbiology and Applied Sciences 6 (6): 2603–2610.
  12. De Riek J, Calsyn E, Everaert I, Van Bockstaele E, De Loose M, 2001. AFLP based alternatives for the assessment of distinctness, uniformity and stability of sugar beet varieties. Theoretical and Applied Genetics 103: 1254–1265.
  13. Frank ham R, Ballou SEJD, Briscoe DA, Ballou JD, 2002. Introduction to conservation genetics. Cambridge university press.
  14. Ganopoulos I, Xanthopoulou A, Molassiotis A, Karagiannis E, Moysiadis T, Katsaris P, Aravanopoulos F, Tsaftaris A, Kalivas A, Madesis P, 2015. Mediterranean basin Ficus carica L.: from genetic diversity and structure to authentication of a Protected Designation of Origin cultivar using microsatellite markers. Trees, 29, pp.1959-1971.
  15. Giraldo E, Lopez-Corrales M, Hormaza JI, 2010. Selection of the Most Discriminating Morphological Qualitative Variables for Characterization of Fig Germplasm. Journal of the American Society for Horticultural Science 135 (3): 240–249.
  16. Girgel U, 2021. Principle component analysis (PCA) of bean genotypes (Phaseolus vulgaris L.) concerning agronomic, morphological and biochemical characteristics. Applied Ecology and Environmental Research 19 (3): 1999–2011.
  17. Hssaini L, Charafi J, Razouk R, Hernandez F, Fauconnier M, Ennahli S, Hanine H, 2020. Assessment of Morphological Traits and Fruit Metabolites in Eleven Fig Varieties (Ficus carica L.). International Journal of Fruit Science 20 (S2): S8–S28. http://doi:10.1080/15538362.2019.1701615
  18. Igwe DO, Ihearahu OC, Osano AA, Acquaah G, Ude GN, 2022. Assessment of genetic diversity of Musa species accessions with variable genomes using ISSR and SCoT markers. Genetic Resources and Crop Evolution 69: 49–70.
  19. Ikegami H, Habu T, Mori K, Nogata H, Hirata C, Hirashima K, Tashiro K, Kuhara S., 2013. De novo sequencing and comparative analysis of expressed sequence tags from gynodioecious fig (Ficus carica L.) fruits: caprifig and common fig. Tree Genetics & Genomes 9 (4): 1075–1088.
  20. Ikegami, H, Nogata H, Hirashima K, Awamura M, Nakahara T, 2009. ‘Analysis of genetic diversity among European and Asian fig varieties (Ficus carica L.) using ISSR, RAPD, and SSR markers’, Genetic Resources and Crop Evolution, 56(2), pp. 201–209. doi: 10.1007/s10722-008-9355-5.
  21. IPGRI C, 2003. Descriptors for fig. International Plant Genetic Resources Institute, Rome, Italy, and International Centre for Advanced Mediterranean Agronomic Studies, Paris, France 52.
  22. Khadivi A, Anjam R, Anjam K, 2018. Morphological and pomological characterization of edible fig (Ficus carica L.) to select the superior trees. Scientia Horticulturae 238: 66–74.
  23. Khodaee L, Azizinezhad R, Etminan AR, Khosroshahi M, 2021. Assessment of genetic diversity among Iranian Aegilops triuncialis accessions using ISSR, SCoT, and CBDP markers. Journal of Genetic Engineering and Biotechnology, 19(1), pp.1-9.
  24. Kumar J, Agrawal V, 2019. Assessment of genetic diversity, population structure and sex identification in dioecious crop, Trichosanthes dioica employing ISSR, SCoT and SRAP markers. Heliyon 5 (3): e01346. http://doi:10.1016/j.heliyon.2019.e01346
  25. Lata H, Chandra S, Techen N, Khan IA, ElSohly MA, 2010. Assessment of the genetic stability of micropropagated plants of Cannabis sativa by ISSR markers. Planta Medica 76 (01): 97–100.
  26. Louati M, Ucarli C, Arikan B, Ghada B, Salhi Hannachi A, Turgut-Kara N, 2019. Genetic, morphological, and biochemical diversity of argan tree (Argania spinosa L.) (Sapotaceae) in Tunisia. Plants, 8(9), p.319.
  27. Manyasa EO, Silim SN, Christiansen JL, 2009. ‘Variability patterns in Ugandan pigeonpea landraces.’, Journal of SAT Agricultural Research, 7, pp. 1–9.
  28. Mei, Z., Zhang, C., Khan, M.A., Zhu, Y., Tania, M., Luo, P. and Fu, J., 2015. Efficiency of improved RAPD and ISSR markers in assessing genetic diversity and relationships in Angelica sinensis (Oliv.) Diels varieties of China. Electronic Journal of Biotechnology, 18(2), pp.96-102.
  29. Mohamed AAH, Nagaty MA, El-Baghdady M, Radwan KH, 2017. Morphological and molecular characterization of some olive (Olea europaea) cultivars in El-Arish, Egypt. Journal of Bioscience and Applied Research 3 (4): 237–251.
  30. Pereira C, Serradilla MJ, Perez-Gragera F, Martin A, Villalobos MC, Lopez-Corrales M, 2017. Evaluation of agronomic and fruit quality traits of fig tree varieties (Ficus carica L.) grown in Mediterranean conditions. Spanish Journal of Agricultural Research, 15(3), pp.e0903-e0903.
  31. Pritchard JK, Stephens M, Donnelly P, 2000. ‘Inference of population structure using multilocus genotype data’, Genetics, 155(2), pp. 945–959. doi: 10.1093/genetics/155.2.945.
  32. Rasul KS, Grundler FMW, Tahir NA, 2022. Genetic diversity and population structure assessment of Iraqi tomato accessions using fruit characteristics and molecular markers. Horticulture, Environment, and Biotechnology 63 (4): 523–538.
  33. Rout GR, Aparajita S, 2009. ‘Genetic Relationships among 23 Ficus Accessions Using Inter-’, Journal of Crop Science and Biotechnology, 12(2), pp. 91–96.
  34. Salhi-Hannachi A, Trifi M, Zehdi S, Hedfi J, Mars M, Rhouma A, Marrakchi M, 2004. Inter-Simple Sequence Repeat fingerprints to assess genetic diversity in Tunisian fig (Ficus carica L.) germplasm. Genetic Resources and Crop Evolution, 51(3), pp.269-275.
  35. Sharifova SS, Mehdiyeva SP, Abbasov MA, 2017. Analysis of genetic diversity among different tomato genotypes using ISSR DNA marker. Genetika 49 (1): 31–42.
  36. Sheikh ZN, Sharma V, Shah RA, Sharma N, Summuna B, Al-Misned FA, El-Serehy HA, Mir JI, 2021. Genetic diversity analysis and population structure in apricot (Prunus armeniaca L.) grown under north-western himalayas using ISSR markers. Saudi Journal of Biological Sciences, 28(10), pp.5986-5992.
  37. Simsek M, Gulsoy E, Kirar MZ, Turgut Y, Yucel B., 2017. Identification and selection of some female fig (Ficus carica L.) genotypes from Mardin province of Turkey. Pakistan Journal of Botany 49 (2): 541–546.
  38. Tamboli BD, Sawale DD, Jagtap PB, Nimbalkar RU, Teke SR., 2015. Effect of micronutrients on yield and fruit quality of fig on Inceptisol. Indian J Hort 72 (3): 419–422.
  39. Yilmaz A, Ciftci V, 2021. Genetic relationships and diversity analysis in Turkish laurel (Laurus nobilis L.) germplasm using ISSR and SCoT markers. Molecular Biology Reports 48 (5): 4537–4547.
  40. Zhang X, Wang X, Liu L, Wang W, Liu Y, Deng Q, Zhang H, Wang X, Xia H, 2020. Evaluation of the comparative morphological and quality in fig (Sichuan, China) with different colors under different ripening stages. Scientia Horticulturae, 265, p.109256.
  41. Zhou H, Liao J, Xia Y, Teng Y., 2013. Morphological characteristics for classifying evergreen Azalea (Ericaceae) cultivars in China using numerical taxonomy. Pakistan Journal of Botany 45 (2): 593–598.