Document Type : Articles
Authors
Biology Department, College of Science, University of Babylon, Babylon, Iraq
Abstract
Food-borne diseases generally correlate with pathogens, such as bacteria, more than 200 food-borne infections caused by food cause significant public health problems worldwide. A total of 100 samples of milk and milk products were gathered randomly from different supermarkets in AL-Hilla City, Iraq, from November 2022 to February 2023 to detect bacteria contamination in these samples. To study the susceptibility of antibiotic 10 antibiotics from different classes. To test the capacity of Enteropathogenic Escherichia coli to produce biofilms congo red agar was used. All EPEC isolates were tested for the presence of genes encoded to adhesion fimH and iha genes. The results showed that the percentage of positive samples was 72(72%), including 19(19%), 30(30%), 14(14%), and 9(9%) isolated from milk, cheese, Yoghurts and cream, respectively. More types of bacteria isolates were E. coli (32) isolates than Staphylococcus aureus (24), Staphylococcus epidermidis (22), Candida albicans (21), Pseudomonas aurogenosa (18), Bacillus cereus (17), Proteus mirabilis (16), Klebsiella pneumonia (10) and, Enterobacter aeruginosa (6). The prevalence of EPEC was 12(37.4%) including 5(15.6), 3(9.3), and 4(12.5) for Polyvalent 2, Polyvalent 3, and Polyvalent 4, respectively. The antibiotic susceptibility to Enteropathogenic E.coli observed lower resistance against Imipenem and Meropenem in prevalence 12(100%) and 11(91.6%) from 12 isolates, While the study indicated that there is a higher resistance against Ampicillin in prevalence11 (91.6%). There are three types of colonies: strong, intermediate, weak, and no biofilm in prevalence. The result of PCR showed the prevalence of fimH and iha genes was 10(83.3%), and 8(66.6%) respectively.
Keywords
)