Document Type : Articles

Authors

Tikrit University – College of Agriculture – Department of Field Crops, Iraq

Abstract

An experiment was carried out at the Department of Field Crops laboratory, College of Agriculture, University of Tikrit, in order to know the effect of stimulating the stored seeds of triticale  Farah cultivar by α -amylase enzyme  using a complete random design (CRD) in a factorial experiment. The first factor was the storage periods of  6 months, 1.5 years and 2.5 years (S1,S2 and S3) and the second factor was α -amylase enzyme concentrations of 0 (dry seeds), seeds soaked in distilled water, 1 and 2 mg L-1 of the enzyme (C1,C2,C3 and C4). Results showed that increasing the storage period reduces the viability of seeds and their ability to  germinate and seedling characters. The percentage of germination, plumule length, radicale, seedling vigor, plumule dry weight and radical dry weight reduced from 93.5%, 10.68 cm,11.05 cm, 2033.42, 0.1149 g and 0.1231 g in S1 treatment to 83.88%, 7.53cm, 7.5 cm, 1267.1, 0.0977 g and 0.1022 g in S3 treatment. The concentration 1 mg L-1 was superior in germination, plumule length, seedling vigor and plumule dry weight and the values were 92.44, 9.46cm, 1805.78 and 0.1111 g. However the concentration of 2 mg L-1 was superior in radical length and its dry weight. The best interaction was between storage period S1 and enzyme concentration C3 with values of 96.6%, 11.50 cm, 2212.33 and 0.1288 g for germination percentage, plumule length, seedling vigor and plumule dry weight and with the concentration C4 that recorded 11.67 cm and 0.1353 g for radical length and dry weight respectively. 

Keywords

  1. Al-Dulaimi, O.Z.A. 2020. Influence of seed variety, activation by salicylic acid and addition of humic acid on growth characters, yield and quality of triticale varieties. Ph.D Thesis, Tikrit University, College of Agriculture.
  2. Al-Tareihi, M.S.M. 2020. Stimulation of deteriorated seeds during storage by alpha- amylase to improve growth and yield in soybean. Ph.D. Thesis, University of Baghdad, College of Agriculture Engineering Sciences.
  3. Azadi, M.S. and E. Younesi 2013. The effects of storage on germination characters and enzyme activity of sorghum seeds. Jour. Of Stress physiol. And Bioch. 9(4):289-298.
  4. Cheyed, S.H. 2020. Effect of storage method and period on vitality and vigor of seed wheat. Indian Jour. Of Eco. Special Issue 47(10) :27-31
  5. Ciornea, E.N., G.C. Vasile and D.C. Cojocaru 2008.On the mobilization of reserve starch under the action of glucanphosphorylase in Panicum miliaceum and Bromus sterilis. Analele Stiintifice ale Universitati Alexandru Ioan Cuza, Sectinuea si Biologie Moleculara.9(2):61-69.
  6. Declerck, N., M. Machius, G. Wiegand, R. Tulber and C. Gaillaridin 2000. Proping structural determinations specifying high Thermo stability in Bacillus linhenif ermis- amylase. Jour. Mol.Biol.301:1041-1057.
  7. Ghassemi-Golezani, K., S. Khomari, B. Dalili, B. Hosseinzadeh-Mahootchy and A. Chadordooz-Jedi 2010. Effect of seed aging on field Performance of winter oil seed rap. Jour.Food Agric.Envir.8(1):175-178.
  8. ISTA (International Seed Testing Assosiation) 2013. Handbook of vigour test method
  9. rd ed. International seed testing association (ISTA), Zurich. Pp.117.
  10. Jyoti, J., N. Lal and A. Kaushik 2011. Partial purification characterization of an α- amylase fron Bacillus licheniformis JAR-26. IJABR2(3):315-320.
  11. Khalil, N.A., A.A. Al-Mitwaly, M.M. Shafik and Al- Morshidy, W.A. 2015. Cereals and Legumes Crops. College of Agriculture, Cair, University, 1st Press, 83-84.
  12. Moyo, R., E. Ndlovu, N. Moyo, S. Kudita and M.Maphose 2015. Physiological parameters of seed vigor in exsitu stored sorghum Germplasm. Jour. Of cereal and oil seeds.6(6):31-38
  13. Murti, G.S.R., G.S. Sirohi and K.K. Upreti 2004. Glossary of plant physiology. Daya
  14. Publishing House. Delhi. Pp.207.
  15. Pandey, A., P. Nigam, C.R. Soccal, V.T. Soccal, D. Shngh and R. Mohan 2000 Advances in Microbial amylase. Biotechnology Appl. Biochem.31:135-152.
  16. Said, M.K.H., 2019. Effect of the wheat seeds storage method and period severity of infestation by Khapra Beetle and Red flour Beetle. M.Sc. Dissertation, College of Graduate Studies, Sudan Univ. of Sci. Tech. Pp:105
  17. Sibande, G.A.K., V.H. Kabambe, M.F. Maliro and V. Karoshi 2015. Effect of priming
  18. technique and seed storage period on soybean (Glysine max L.) germination. Jour
  19. of Dynamics in Agric. Res. 2(5):46-53.
  20. Tilebei, H.G. and A. Golpayegani 2011. Effect of seed ageing on physiological and biochemical changes in rice seed (Orysa sativa L.) Int.Jour.Agric.Sci.1(3):138- 143
  21. Tiwari, R.K.S. and K.Das 2014. Impact of differential storage conditions on seed germination and viability of some medicinal plants. African Jour. Agric. Res. 9(20)1578-1585.