Document Type : Articles
Authors
1 Department of Plant Protection- College of Agriculture-Tikrit University
2 Department of Biology-College of Sciences- Tikrit University
Abstract
This study was carried out to study some morphological parts of adult male of Liriomyza spp, including the antenna, the femur and the middle vein (Cu Al), as well as the shape of mines that larvae made them on the leaves. Results showed that there were two species of L.bryoinae on tomato crop and L.sativae on climbing beans crop, this was agreed with the molecular study that it was conducted using the RAPD Random Amplification of Polymorphic DNA based on the PCR technique to determine genetic variability of DNA that it was extraction from adults and pupae and obtained a quantity of it ranged from (35-556.9) mg and purity ranged between (1.7-2).
The RAPD markers were studied using 19 random primers, 13 of which showed a bands and 6 of them didn’t show any bands, the total number of bands loci were 53 loci, 23 of which were main bands and 30 were polymorphism and genetic analysis was performed based on these results. The genetic distance was found and lowest was (0.125) among adults and pupae of bean crop and higher distance was (0.323) the among the adults and the pupae of the tomato crop and the genetic distance between the tomato pupae and bean pupae was (0.185), according to this values the genetic relationship was found which was the tomato adult's species was independent and the genetic relationship between Adults and pupae have closest. Results showed that the pupae which were taken from the crops, there are two species of Liriomyza spp .
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