Document Type : Articles
Authors
Department of Animal Sciences, College of Agricultural Engineering Sciences, University of Sulaimani, Iraq.
Abstract
This study aimed to evaluate the effect of repeated freeze-thaw cycles on some electrolytes’ concentration (Na+, K+, Ca+ and Cl−) and thyroid hormones (T3 and T4), in the serum of Karadi sheep breed. This study was conducted on 18 male and female sheep (aged over one year) in two lines of Karadi sheep Jeshana and Jaff in Sulaimaniyah province. Blood samples were collected from each animal and allowed to clot for 45 to 60 min, then centrifuged at 3000 × g for 10 min to separate the serum. The serum sample was frozen at (-40) degrees and was monitored for 18 months. Serum sample analyzed after (18 months) of storage. Our result showed that repeated freeze-thaw cycling has significant and relevant increases of serum T4 (Thyroxine hormone) in both male and female groups without affecting T3 (Triiodothyronine hormone). Na+ and Cl- in both male and female groups showed a significant difference compared to the control group while the remaining electrolytes K+ and Ca+ didn’t show any relevant changes. This study has demonstrated that repeated freeze-thaw cycles do not cause changes in some biochemical constituents studied in sheep serum.
Keywords
- Animal Production and Veterinary Directorate (2011). 8th Edn., 18:23.
- Bishop, M.L., Fody, E.P. and Schoeff, L.E. (2010). Clinical chemistry: techniques, principles correlations. Philadelphia: Library of Congress.
- Blaszczyk, B., Udala, J. and Gaczarzewicz, D. (2004). Changes in estradiol, progesterone, melatonin, prolactin, and thyroxine concentrations in the blood plasma of goats following induced estrus in and outside the natural breeding season. Small. Rumin. Res., 51(3): 209-219.
- Boyanton, B.L. and Blick, K.E. (2002). Stability studies of twenty-four analytes in human plasma and serum. Clin. Chem., 48(12): 2242–2247.
- Clark, S., Youngman, L.D., Palmer, A., Parish, S., Peto, R. and Collins, R. (2003). Stability of plasma analytes after delayed separation of whole blood: implications for epidemiological studies. Int. J. Epidemiol., 32(1): 125-130.
- Comstock, G.W., Burke, A.E., Norkus, E.P., Gordon, G.B., Hoffman, S.C. and Helzlsouer, K.J. (2001). Effects of repeated freeze-thaw cycles on concentrations of cholesterol, micronutrients, and hormones in human plasma and serum. Clin. Chem., 47(1): 139-142.
- Cray, C., Rodriguez, M., Zaias, J. and Altman, N.H. (2009). Effects of storage temperature and time on clinical biochemical parameters from rat serum. J Am Assoc Lab Anim Sci., 48(2): 202-204.
- Cuhadar, S., Koseoglu, M., Atay, A. and Dirican, A. (2012). The effect of storage time and freeze-thaw cycles on the stability of serum samples. Biochem. Med. (Zagreb), 23(1): 70-77.
- Fox, C.S., Pencina, M.J., D’Agostino, R.B., Murabito, J.M., Seely, E.W., Pearce, E.N. and Vasan, R.S. (2008). Relations of thyroid function to body weight: cross-sectional and longitudinal observations in a community-based sample. Arch. Intern. Med., 168(6): 587–592.
- Gislefoss, R.E., Lauritzen, M., Langseth, H. and Morkrid, L. (2017). Effect of multiple freeze-thaw cycles on selected biochemical serum components. Clin. Chem. Lab. Med., 55(7): 967-73.
- Hawkins, M.G., Kass, P.H., Zinkl, J.G. and Tell, L.A. (2006). Comparison of biochemical values in serum and plasma, fresh and frozen plasma, and hemolyzed samples from orange-winged amazon parrots (Amazona amazonica). Vet. Clin. Pathol., 35(2): 219–225.
- Heins, M., Heil, W. and Withold, W. (1995). Storage of serum or whole blood samples? Effects of time and temperature on 22 serum analytes. Eur. J. Clin. Chem. Clin. Biochem, 33(4): 231–238.
- Hillebrand, J.J., Heijboer, A.C. and Endert E. (2017). Effects of repeated freeze-thaw cycles on endocrine parameters in plasma and serum. Ann. Clin. Biochem., 54(2): 289-292.
- Houston, M.C., and Harper, K.J. (2008). Potassium, magnesium, and calcium: their role in both the cause and treatment of hypertension. J. Clin. Hypertens. (Greenwich), 10 (7 Suppl 2): 3–11.
- Hu, K., Stewart, A. J., Yuen, K. Y., Hinrichsen, S., Dryburgh, E. L. and Bertin, F. R. (2020). The effect of freeze-thaw cycles on determination of immunoreactive plasma adrenocorticotrophic hormone concentrations in horses. J. Vet. Int. Medic., 34(3): 1350–1356.
- Ismail, A.A. (2017). The effects of repeated freeze–thaw cycles on endocrine parameters in plasma and serum. Ann. Clin. Bioch., 54(5): 622-623
- Iwen, K.A., Schroder, E. and Brabant, G. (2013). Thyroid hormone and the metabolic syndrome. Eur. Thyroid. J., 2(2): 83–92
- Kashiwai, T., Ichihara, K., Tamaki, H., Endo, Y., Kimura, M., Takeoka, K. et al. (1991). The stability of immunological and biological activity of human thyrotropin in buffer: its temperature-dependent dissociation into subunits during freezing. Scand. J. Clin. Lab. Invest., 51(5): 417-423.
- Knudsen, N., Laurberg, P., Rasmussen, L.B., Bulow, I., Perrild, H., Ovesen, L. and Jorgensen, T. (2005). Small differences in thyroid function may be important for body mass index and the occurrence of obesity in the population. J. Clin. Endocrinol. Metab., 90(7): 4019 – 4024.
- Koliakos, G., Gaitatzi, M. and Grammaticos, P. (1999). Stability of serum TSH concentration after non refrigerated storage. Panminerva. Med., 41(2): 99 –101.
- Männistö, T., Surcel, H.M., Bloigu, A., Ruokonen, A., Hartikainen, A.L., Jarvelin, M.R. et al. (2007). The effect of freezing, thawing, and short- and long-term storage on serum thyrotropin, thyroid hormones, and thyroid autoantibodies: implications for analyzing samples stored in serum banks. Clin. Chem., 53(11):1986-1987.
- Männistö, T., Suvanto, E., Surcel, H.M. and Ruokonen, A. (2010). Thyroid hormones are stable even during prolonged frozen storage. Clin. Chem. Lab. Med., 48(11): 1669-70
- Mcpherson, R.A. and Pincus, M.R. (2011). Henry’s clinical diagnosis and management by laboratory methods. New York: Elsevier.
- Najat, D. (2017). Prevalence of PreAnalytical Errors in Clinical Chemistry Diagnostic Labs in Sulaimani City of Iraqi Kurdistan. PLoS ONE, 12(1): e0170211.
- Nixon, D.A., Akasha, M.A. and Anderson, R.R. (1988). Free and total thyroid hormones in serum of Holstein cows. J. Dairy. Sci., 71(5): 1152-1160.
- Nwankpa, P., Ekweogu, C.N., Emengaha, F.C., Ugwuezumba, P., Chukwuemeka, O.G., Etteh, C.C. et al. (2018). Influence of freeze – thaw and storage time on some specific human hormones. Niger. J. Exp. Clin. Biosci., 6(2): 33-36.
- Oddie, T.H., Klein, A.H., Foley, T.P. and Fisher, D.A. (1979). Variation in values for iodothyronine hormones, thyrotropin, and thyroxine-binding globulin in normal umbilical-cord serum with season and duration of storage. Clin. Chem., 25(7): 1251-1253.
- Oddoze, C., Lombard, E. and Portugal, H. (2012). Stability study of 81 analytes in human whole blood, in serum and in plasma. Clin. Biochem., 45(6): 464–469.
- Paltiel, L., Rønningen, K.S., Meltzer, H.M., Baker, S.V. and Hoppin, J.A. (2008). Evaluation of Freeze Thaw Cycles on stored plasma in the Biobank of the Norwegian Mother and Child Cohort Study. Cell. Preserv. Technol., 6(3): 223-230.
- Reyna, R., Traynor, K.D., Hines, G., Boots, L.R. and Azziz, R. (2001). Repeated freezing and thawing does not generally alter assay results for several commonly studied reproductive hormones. Fertil. Steril., 76(4): 823-825.
- Reynolds, B., Taillade, B., Medaille, C., Palenche, F., Trumel, C. and Lefebvre, H.P. (2006). Effect of repeated freeze-thaw cycles on routine plasma biochemical constituents in canine plasma. Vet. Clin. Pathol., 35(3): 339-340.
- Riis, P.M. and Madsen, A. (1985). Thyroxine concentration and secretion rates in relation to pregnancy, lactation and energy balance in goats. J. Endo., 107(3): 421–427.
- Thoresen, S.I., Havre, G.N., Morberg, H. and Mowinckel, P. (1992). Effects of storage time on chemistry results from canine whole blood, heparinized whole blood, serum and heparinized plasma. Vet. Clin. Pathol., 21(3): 88–94.
- Thoresen, S.I., Tverdal, A., Havre, G. and Morberg, H. (1995). Effects of storage time and freezing temperature on clinical chemical parameters from canine serum and heparinized plasma. Vet. Clin. Pathol., 24(4): 129-133.
- Todini, L., A. Malfatti, A. Valbonesi, M. Trabalza-Marrinucci and A. Debenedetti. (2007). Plasma total T3 and T4 concentrations in goats at different physiological stages, as affected by the energy intake. Small. Rumin. Res., 68(3): 285-290.
- Vlot, M.C., den Heijer, M., de Jongh, R.T., Vervloet, M.G., Lems, W.F., de Jonge, R., Obermayer-Pietsch, B. and Heijboer, A.C. (2018). Clinical utility of bone markers in various diseases. Bone., 114: 215–225.
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