Document Type : Articles

Authors

Food Sciences Dept., College of Agriculture, University of Tikrit, Tikrit, Iraq.

Abstract

The current study was conducted in the laboratories of the College of Agriculture and Animal House of the College of Veterinary Medicine at Tikrit University for the period from 5/5/2020 to 29/10/2020. And it aimed to produce therapeutic food mixtures to treat people with malnutrition and to know their impact on the health of the small intestine and large intestine. Where three ready-to-use therapeutic food mixtures have been prepared (do not need to be prepared or cooked before eating) consisting of milk, sprouted oats, non-sprouted oats, Sesame, Rice, chickpeas, Dates, bananas, apples, potatoes and oil, provided that the moisture and fat content does not exceed 2.5% and 32%, the percentage of protein and carbohydrates is not less than 14% and 40% respectively, and they were given to Thirty healthy rats and divided into six groups equally, a control group (+) natural, control group (-) (malnourished for a period of time 48 days), 1st, 2nd, 3d and 4th. The studied groups were affected with malnutrition for a period of 24 days, then the first food mixture was given to the 1st, the second food mixture for the 2nd, the third food mixture for 3d, the standard food mixture F-100 for the 4th. After 24 days of consuming the prepared nutritional mixtures, the tissues of the small and large intestine were examined under a microscope. The histological analysis of the small intestine showed that the 1st and the 2nd gave completely healthy tissues to the small intestine similar to the control group (+) and the 4th, while the 3d showed congestion in the blood vessels with hyperplasia of intestinal epithelial cells compared to the control group (-) which showed High-intensity cellular degeneration in the lining cells of the intestine with chronic and acute inflammations. As for the large intestine, the 1st gave a complete recovery of colon tissue, similar to the control group (+) and the 4th, while the 2nd and 3d showed pathological changes that included infiltration and local grouping of inflammatory cells in the submucosal layer of the colon and flattening of the villi and their union with acute inflammation compared to a control group (- ), Which showed pathological changes represented by infiltration of inflammatory cells with an overgrowth of mesenteric lymph tissue and multiple lymph sacs. It was found that the first nutritional mixture prepared on which the 1st of rats fed give similar results to the results of the control group (+) and the 4th

Keywords

  1. Attia, S., Feenstra, M., Swain, N., Cuesta, M. and Bandsma, R. H. (2017). Starved guts: morphologic and functional intestinal changes in malnutrition. Journal of pediatric gastroenterology and nutrition, 65(5), 491-495.‏
  2. Bancroft, J. and Steven, A. (1982). Theory and practice of histological techniques, 2nd ed., Churchill Livingstone, London, No 109-120.
  3. Bati, B., Celik, I. and Dogan, A. (2015). Determination of hepatoprotective and antioxidant role of walnuts against ethanol-induced oxidative stress in rats. Cell biochemistry and biophysics, 71(2), 1191-1198.‏
  4. Bibi, S., Navarre, D. A., Sun, X., Du, M., Rasco, B. and Zhu, M. J. (2019). Beneficial effect of potato consumption on gut microbiota and intestinal epithelial health. American Journal of Potato Research, 96(2), 170-176.‏
  5. Borg, B., Mihrshahi, S., Laillou, A., Sigh, S., Sok, D., Peters, R. and Wieringa, F. T. (2019). Development and testing of locally-produced ready-to-use therapeutic and supplementary foods (RUTFs and RUSFs) in Cambodia: lessons learned. BMC public health, 19(1), 1-9.‏
  6. Chatterton, D. E., Nguyen, D. N., Bering, S. B. and Sangild, P. T. (2013). Anti-inflammatory mechanisms of bioactive milk proteins in the intestine of newborns. The international journal of biochemistry & cell biology, 45(8), 1730-1747.‏‏
  7. Das, S. H. O. B. H. A., Yadav, R. K. and Nagchoudhuri, J. (2004). Effect of protein malnutrition on the intestinal absorption of monosaccharides in rats in vivo. Indian journal of physiology and pharmacology, 48(1), 96-100.
  8. Elhardallou, S. B., Farh, S. G. E. M. and Gobouri, A. A. (2015). Production, Storage and Evaluation of Homemade and Processed Diet, Based on Wheat, Legumes, Sesame and Dates; for Under-Five Children.‏
  9. Hajimohammadi, A., Mottaghitalab, M. and Hashemi, M. (2020). Effects of microbial fermented sesame meal and enzyme supplementation on the intestinal morphology, microbiota, pH, tibia bone and blood parameters of broiler chicks. Italian Journal of Animal Science, 19(1), 457-467.‏
  10. HE, B., Bai, Y., Jiang, L., Wang, W., Li, T., Liu, P. and Wang, J. (2018). Effects of oat bran on nutrient digestibility, intestinal microbiota, and inflammatory responses in the hindgut of growing pigs. International journal of molecular sciences, 19(8), 2407.‏
  11. Krapf, J., Kandzia, F., Brühan, J., Walther, G. and Flöter, E. (2019). Sprouting of oats: A new approach to quantify compositional changes. Cereal Chemistry, 96(6), 994-1003.‏
  12. Nadeem, M., Qureshi, T. M., Ahmad, M. M., Riaz, M. N. and Ameer, A. (2017). Development of free flowing date powder and its utilization im muffins to enhunce nutritional value. Journal of Agricultural Research (03681157), 55(4).‏
  13. Nakajima, V., Kobayasi, S., Naresse, L. E., Leite, C. V. D. S., Curi, P. R. and Montovani, J. C. (2008). Alterations in the intestinal wall due to protein malnutrition in rats: evaluation of the rupture strength and the tissue's collagen. Acta cirurgica brasileira, 23(5), 435-440.‏
  14. NRC, National Research Council (1995). Nutrient Requirements of Laboratory Animals 4th ed. National Academy Press,Washington, DC.
  15. Rolfes, S. R., Pinna, K. and Whitney, E. (2020). Understanding normal and clinical nutrition. Cengage learning.‏
  16. Salameh, E., Morel, F. B., Zeilani, M., Dechelotte, P. and Marion-Letellier, R. (2019). Animal models of undernutrition and enteropathy as tools for assessment of nutritional intervention. Nutrients, 11(9), 2233.‏
  17. Sharoba, A. M. (2014). Nutritional value of spirulina and its use in the preparation of some complementary baby food formulas. Journal of Food and Dairy Sciences, 5(8), 517-538.‏
  18. Singh, R., Kaushik, R. and Gosewade, S. (2018). Bananas as underutilized fruit having huge potential as raw materials for food and non-food processing industries: A brief review. The Pharma Innovation Journal, 7(6), 574-580.‏
  19. Sobotka, L., and Forbes, A. (2019). Basics in clinical nutrition (Vol. 1, No. 5th). Galen.‏
  20. Tian, D. D., Xu, X. Q., Peng, Q., Zhang, Y. W., Zhang, P. B., Qiao, Y. and Shi, B. (2020). Effects of banana powder (Musa acuminata Colla) on the composition of human fecal microbiota and metabolic output using in vitro fermentation. Journal of Food Science, 85(8), 2554-2564.‏
  21. Unicef, (2020). Children make up the majority of up to 4.5 million Iraqis at risk of falling into poverty and deprivation due to the impact of covid-19.