Document Type : Articles

Authors

1 Department of Animal Science, College of Agricultural Engineering Sciences, University of Sulaimani, Iraq

2 College of Veterinary Medicine, University of Sulaimani, Iraq

Abstract

This study aimed to demonstrate how different fish weights and clove powder concentration levels together influence anesthesia and recovery time in Cyprinus carpio. Two different body weights classes of common carp (45±5g and 95±5g) were subjected to three concentrations of clove powder (200, 300 and 400mg/L) each replicated four times. At each treatment combination, the anesthetic induction and recovery times were recorded, fish held for a period of one week to two months following the experiments to assess short-term and long-term mortality. Significant differences (P<0.05) appeared in the effect of combination between the different levels of clove powder (mg/L) with the two different weight common carp (C. carpio L.) on anesthesia time, but there was no difference in recovery time between the two size groups. The induction time was less than one minute for doses of 300 and 400 mg/L in lower fish weight 45±5g while in 200mg/L dose with 95±5g of fish weight it needed more than 9 minutes. At all tested concentrations, induction time was significantly weight-dependent (p< 0.05), but the recovery time was not. Significant alterations were recorded in the hematological variables of the fish (RBC, HB, MCH and WBC), no mortality was observed. This study has demonstrated that clove powder can be safely and effectively use in the anesthesia of common carp and anesthesia time affect by doses of clove powder with fish weight.

Keywords

  1. Abdolazizi, S., Naghdi, N. and Kamaya, B. (2011). Effect of clove oil as an anesthetic on some haematological parameters of Carassius auratus. Aqua. Rese. Devel., 2: 1-3.
  2. Akinrotimi, O.A., Abu, O.M.G., Bekibele, D.O., Uedene–Naa, B., Aranyo, A.A. (2010). Haematological characteristics of Tilapia guineensis from Buguma Creek, Niger Delta, Nigeria. Elec. J. Envi., Agri. Food Chemi., 9(8): 1415-1422.
  3. Akinrotimi, O.A., Gabriel, U.U. and Edun, O.M. (2015). The efficacy of clove seed extracts as an anaesthetic agent and its effect on haematological parameters of African catfish (Clarias Gariepinus). Int. J. Aqua. Fis. Sci., 1(2): 042-047.
  4. Al-Jashami, K.J., Al-Biaty, M.N., Al-Obaedy, H.J. and Al-Jebury, A.G. (2002). New anesthetic for common carp (Cyprinus carpio L.) fish. Ira. J. Agri., 7(1): 111-116 (In Arabic).
  5. Aydın, I., Akbulut, B., Küçük, E. and Kumlu, M. (2015). Effects of temperature, fish size and dosage of clove oil on anesthesia in turbot (Psetta maxima Linnaeus, 1758). Tur. J. Fis. Aqua. Sci., 15(4): 899-904.
  6. Davis, M.W. (2010). Fish stress and mortality can be predicted using reflex impairment. Fis. Fis., 11(1): 1–11.
  7. Farahi, A., Kasiri, M., Sudagar, M. and Iraei, M.S. (2011). Size-relative effectiveness of clove essence as an anesthetic for kutum (Rutilus frisii kutum). Glo. Vet., 6(2): 180-184.
  8. Fast, M.D., Hosoya S., Johnson S.C. and Afonso L.O.B. (2008). Cortisol response and immune- related effects of Atlantic salmon (Salmo salar Linnaeus) subjected to short- and long-term stress. Fis. Shellfis. Immunol, 24(2): 194-204.
  9. Gholipourkanani, H., Mirzargar, S.S., Soltani, M., Ahmadi, M., Abrishamifar, A., Bahonar, A. and Yousefi, P. (2011). Anesthetic effect of tricaine methanesulfonate, clove oil and electroanesthesia on lysozyme activity of Oncorhynchus mykiss. Iran. J. Fis. Sci., 10(3): 393-402.
  10. Hassan, B.R. (2016). Impact of Clove and Mustard as Anesthetics on Small Common Carp (Cyprinus carpio L.). College of Agricultural Engineering Sciences, University of Sulaimani, Msc Thesis, 102pp.
  11. Hoseini, S.M. and Ghelichpour, M. (2011). Efficacy of clove solution on blood sampling and hematological study in Beluga, Huso huso L. Fis. Phy. Bioch., 38(2): 493–498.
  12. Hoseini, S.M. (2011). Efficacy of clove powder solution on stress mitigation in juvenile common carps, (Cyprinus carpio L.). Com. Clin. Path., 20(4): 359–362.
  13. Hoseini, S.M., Rajabiesterabadi, H. and Tarkhani, R. (2013). Anaesthetic efficacy of eugenol on iridescent shark, Pangasius hypophthalmus (Sauvage, 1878) in different size classes. Aqua. Rese., 46(2): 405-412.
  14. Husen, A. and Sharma, S. (2014). Efficacy of anesthetics for reducing stress in fish during aquaculture practices- a review. J. Sci., Engi. Tech., 10(1): 104–123.
  15. Iheanacho, S.C., Ogunji, J.O., Ogueji, E.O., Nwuba, L.A., Nnatuanya, I.O., Ochang, S.N., Mbah, C.E., Usman, I.B., and Haruna, M. (2017). Comparative assessment of ampicillin antibiotic and ginger (Zingiber officinale) effects on growth, haematology and biochemical enzymes of Clarias gariepinus Juvenile. J. Phar. Phy., 6(3): 761-767.
  16. Imanpoor, M.R., Bagheri, T. and Hedayati, S.A.A. (2010). The Anesthetic Effects of Clove Essence in Persian Sturgeon (Acipencer persicus). Wor. J. Fis. Mari. Sci., 2(1): 29-36.
  17. Jahanbakhshi, A., Shaluei, F., Baghfalaki, M., Ramazi, F.G. and Ahmadvand, S. (2012). Efficacy of 2 phenoxyethanol as an Anesthetic for Two Size of Persian Sturgeon, Acipenser persicus. Walailak J. Sci. Tech., 9(1): 31‐36.
  18. Kaps, M. and Lamberson, W.R. (2004). Biostatistics for animal science. CABI Publishing, pp. 294-312.
  19. Maricchiolo, G. and Genovese, L. (2011). Some contributions to the knowledge of stress response in innovative species with a particular focus on the use of anesthetics. Th. Op. Mari. Bio. J., 5(1): 24–33.
  20. Mohammadizarejabad, A., Bastami, K.D., Sudagar, M. and Pourali-Motlagh, S. (2009). Hematology of great sturgeon (Huso huso Linnaeus, 1758) juvenile exposed to clove powder as an anesthetic. Comp. Clini. Path., 19(5): 465-468.
  21. Mylonas, C.C., Cardinaletti, G., Igelaki, I. and Polzonetti, M. (2005). Comparative efficacy of clove oil and 2-phenoxyethanol as anaesthetics in the aquaculture of European sea bass (Dicentrarchus labrax) and gilthead sea bream (Sparus aurata) at different temperatures. Aqua. Rese., 246(4): 467-481.
  22. Okey, I.B. (2019). Effects of clove (Eugenia carophylatta) powder anaesthetic on some haematological and biochemical parameters of Heterobranchus bidorsalis Juveniles. J. Agri. Aqua., 1(1): 1-14
  23. Okey, I.B., Keremah, R.I. and Gabriel, U.U. (2018). The efficacy of clove (Eugenia caryophyllata) powder as anaesthesia on African catfishes (Clarias gariepinus and Heterobranchus bidorsalis) fingerlings. J. Aqua. Mari. Bio., 7(4): 182‒188.
  24. Ross, L.G. and Ross, B. (2008). Anaesthetic and sedative techniques for aquatic animals. 3rd Edition. Blackwell publishing, pp. 7-21
  25. Santos, S., Ghanawi, J. and Saoud, P. (2013). Effects of water temperature and body weight on anaesthetic efficiency in marbled rabbitfish (Siganus rivulatus). Aqua. Rese., 46(4): 928-936.
  26. Shahi, J. and Singh, A. (2011). Effect of bioactive compounds extracted from euphorbious plants on hematological and biochemical parameters of Channa punctatus. Revista Insti. Medic. Trop. São Paulo, 53(5): 259-63.
  27. Sudagara, M., Mohammadizarejabada, A., Mazandarania, R. and Pooralimotlagha. S. (2009). The efficacy of clove powder as an anesthetic and its effects on hematological parameters on roach (Rutilus rutilus). J. Aqua. Feed Sci. Nutri., 1(1): 1-5.
  28. Summerfield, R.C. and Smith, L.S. (1990). Anesthesia, surgery and related tecniques. In: Schreck CB, Moyle PB, Eds. Methods in Fish Biology. Bethesda. American Fisheries Society, pp. 213-272.
  29. Taylor, P.W. and Roberts, S.D. (1999). Clove oil: an alternative anesthetic for aquaculture. North Americ. J. Aqua., 61(4): 150-155.
  30. Tsantilas, H., Galatos, A.D., Athanassopoulou, F., Prassinos, N.N. and Kousoulaki, K. (2006). Efficacy of 2-phenoxyethanol as an anaesthetic for two size classes of white sea ream, Diplodus sargus L., and sharp snout sea bream, Diplodus puntazzo C. Aqua., 253(1): 64–70.
  31. Walsh, C.T. and Pease, B.C. (2002). The use of clove oil as an anaesthetic for the longfinned eel, Anquilla reinhardtii (Steindachner). Aqua. Rese., 33(8): 627–635.
  32. Weber, R.A., Peleteriro, J.B., Garcia-Martin, I.O. and Aldegonde, M. (2009). The efficacy of 2-phenoxyethenol metomidate, clove oil and MS-222 as anaesthetics agents in the Senegalese Sule. Aqua., 288(2): 147-150.
  33. Woody, C.A., Nelson, J. and Ramstad, K. (2002). Clove oil as an anaesthetic for adult sockeye salmon, field trials. J. Fish Bio., 60(2): 340-347.
  34. Zahl, I.H., Kiessling, A., Samuelsen, O.B. and Hansen, M.K. (2009). Anaesthesia of Atlantic cod (Gadus morhua) - effect of pre-anaesthetic sedation, and importance of body weight, temperature and stress. Aqua., 295(1-2): 52–59.
  35. Zahl, I.H., Kiessling, A., Samuelsen, O.B. and Hansen, M.K. (2011). Anaesthesia of Atlantic halibut (Hippoglossus hippoglossus)–effect of pre-anaesthetic sedation, and importance of body weight and water temperature. Aqua. Rese., 42(9): 1235–1245.